Mechanism of Action of Biotin Biotin excerts it`s pharmacological action by influencing metabolic reactions. It acts as a coenzyme for carbohydrate, fat, and protein metabolism. It is required for cell growth and production of fatty acids. It is required for healthy maintenance of hair, skin, sweat glands, nerve tissue, and bone marrow. It also helps in the transfer of carbon dioxide and in the maintenance of a steady blood sugar level